Part:BBa_K3021005:Design

pilA-Laccase-HisTag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 928
  Illegal BglII site found at 1360
  Illegal XhoI site found at 960
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1599
  Illegal AgeI site found at 327
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 882
  Illegal SapI.rc site found at 392
  

Design Notes

The sequence was codon optimized.

Source

Laccase sequence is codon optimized from previous iGEM team, which was initiated based on Trametes versicolor;